DNA Sequencing
Seracare Life Sciences Inc Seraseq ctDNA Complete Mutation Mix AF0.1% | 1x25ul
To further accelerate the validation and clinical application of liquid biopsy-based ccfDNA-targeted NGS assays, SeraCare has developed the Seraseq ctDNA Complete that expands coverage of all relevant variant types including SNVs, INDELs, CNVs, and gene fusions in a single highly-multiplexed reference sample.
Zymo Research Corporation Zymo-Seq RRBS Library Kit (24 preps)
DNA methylation occurs predominantly in CpG contexts, and these CpG dinucleotides are more abundant in select regions of the genome. By enriching for CpG-dense regions and sequencing only the fragments pertaining to those regions, the RRBS platform allows for the capture of a significant amount of methylation data while reducing the amount of sequencing, leading to a substantially decreased cost. This combination makes RRBS the perfect platform for pilot studies. Libraries prepared by Zymo-Seq RRBS™ Library Kit cover 75% CpG islands, 70% gene promoters, 75% gene bodies, and 2.5-4 million unique CpG sites at 5-10x coverage when applied to human samples.
Zymo Research Corporation Zymo-Seq UDI Primer Set (Indexes 1-12)
Zymo-Seq UDI Primer Set (Indexes 1-12)
New England Biolabs, Inc. NEBNext® Enzymatic Methyl-seq Kit – 96 reactions
NEBNext Enzymatic Methyl-seq (EM-seq) is a new method for identification of 5-mC and 5-hmC. While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. In contrast, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.
- Superior sensitivity of detection of 5-mC and 5-hmC
- Greater mapping efficiency
- More uniform GC coverage
- Detection of more CpGs with fewer sequence reads
- Uniform dinucleotide distribution
Zymo Research Corporation RRHP 5-hmC Library Prep Kit 25 preps
The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated with higher frequency of sequencing reads. RRHP bypasses the need for bisulfite conversion, which allows for DNA inputs as low as 100 ng, lower sequencing depth, and straight-forward bioinformatics processing.
Bulldog Bio Inc CleanDTR Dye Terminator Removal Beads, 5ml
CleanDTR is an efficient, paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reactions prior to capillary electrophoresis. The process involves just three simple steps: bind, wash, and elute.
Zymo Research Corporation EZ DNA Methylation-Lightning Kit (CE-IVD), 50 rxns
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
AB Sciex LLC Separation Gel- 10 ml
Separation gel used with the CEQ 8800 and the single rail GeXP instrument.
Zymo Research Corporation Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 1
The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
Seqmatic LLC TAILORMIX DUAL-INDEXED PHIX LI
TAILORMIX DUAL-INDEXED PHIX LIBRARY CONTROL, NON-DENATURED, 10nM, 10uL (12 PACK)